Alzheimer's disease

• Alzheimer's disease is the most common form of dementia among the older people.
• Alzheimer's disease is one of the brain disorder, it leads to memory loss and cognitive decline.

• Alzheimer's disease is neurologically characterized by the formation of senile plaques and neurofibrillary tangles in the brain and blood vessels.

Vascular dysfunction in AD

• Αβ peptides attenuate resting CBF (K Niwa et al., 2001)
• Hemodynamic response decreased with increasing age of the animal in AD model mice (T Mueggler et al., 2003)

Tissue isolation • Basilar artery (BA) was isolated from SD rat • Connective tissue and fat tissue were removed from vessel • Each vessel was incubated with Amyloid peptides for up to 6h.

Cell culture

• A10 (rat embryonic vascular smooth muscle cell) was cultured.
• They were incubated at 37°in 5% CO₂ and O₂ balance with amyloid peptides.


Western blot & Calcium imaging

Immunocytochemistry

To confirm increase of myosin phosphorylation-induced by amyloid beta in A-10 cells, ICC will carried out with confocal microscopy.


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